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Cell Signaling Technology Inc hdac2

(A) Volcano plot showing global protein expression in HL60 cells transduced with P311PP mutant. Blue and red dots show significantly decreased or increased proteins (adjusted p-value<0.05). CoREST components RREB1, RCOR1, KDM1A, RCOR3, and ZNF217 are highlighted in the volcano plot. The global proteome data was plotted after excluding PCDHGA11. (B) High throughput compound screening in HL60 cells stably expressing RCOR1-GFP treated with UM171 (200nM). Schematic of the screening rationale (top panel). The compounds were added at a final concentration of 1µM, and flow analysis was performed after 3 hours post treatment to measure the relative GFP expression (bottom panel). Red dots show the hits from the screen and the table on right-side shows the functional classification of the hits. (C) A dose-titration experiment showing the rescue of RCOR1-GFP by two class I specific HDAC inhibitors (mocetinostat and romidepsin) and three Pan HDAC inhibitors (belinostat, pracinostat and vorinostat). GFP mean fluorescence intensity of DMSO treatment was used as controls. Data from 3 replicates from 1 of 2 independent experiments with similar results are shown. (D) RCOR1 protein levels in P311PP expressing HL60 cells either treated with DMSO, belinostat (320nM) or mocetinostat (300nM) for 3 hours. (E) RCOR1 ELM2-GFP clones were expressed in HL60 cells and analyzed for the interaction with <t>HDAC2</t> through immunoprecipitation. The degradation profiles of the corresponding alanine substitution clones to UM171 treatment are represented as a heat map. (F) Schematic representation of the mechanistic basis of HDAC inhibitors in preventing the CoREST degradation.

Hdac2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hdac2/LSD1+Rabbit+mAb/bio_rxiv__64898__2026__03__12__711277-67-25-29
Average 95 stars, based on 124 article reviews

hdac2 - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "Cancer-associated KBTBD4 mutations induce differentiation defects and confer a unique therapeutic vulnerability"

Article Title: Cancer-associated KBTBD4 mutations induce differentiation defects and confer a unique therapeutic vulnerability

Journal: bioRxiv

doi: 10.64898/2026.03.12.711277

Figure Legend Snippet: (A) Volcano plot showing global protein expression in HL60 cells transduced with P311PP mutant. Blue and red dots show significantly decreased or increased proteins (adjusted p-value<0.05). CoREST components RREB1, RCOR1, KDM1A, RCOR3, and ZNF217 are highlighted in the volcano plot. The global proteome data was plotted after excluding PCDHGA11. (B) High throughput compound screening in HL60 cells stably expressing RCOR1-GFP treated with UM171 (200nM). Schematic of the screening rationale (top panel). The compounds were added at a final concentration of 1µM, and flow analysis was performed after 3 hours post treatment to measure the relative GFP expression (bottom panel). Red dots show the hits from the screen and the table on right-side shows the functional classification of the hits. (C) A dose-titration experiment showing the rescue of RCOR1-GFP by two class I specific HDAC inhibitors (mocetinostat and romidepsin) and three Pan HDAC inhibitors (belinostat, pracinostat and vorinostat). GFP mean fluorescence intensity of DMSO treatment was used as controls. Data from 3 replicates from 1 of 2 independent experiments with similar results are shown. (D) RCOR1 protein levels in P311PP expressing HL60 cells either treated with DMSO, belinostat (320nM) or mocetinostat (300nM) for 3 hours. (E) RCOR1 ELM2-GFP clones were expressed in HL60 cells and analyzed for the interaction with HDAC2 through immunoprecipitation. The degradation profiles of the corresponding alanine substitution clones to UM171 treatment are represented as a heat map. (F) Schematic representation of the mechanistic basis of HDAC inhibitors in preventing the CoREST degradation.

Techniques Used: Expressing, Transduction, Mutagenesis, High Throughput Screening Assay, Stable Transfection, Concentration Assay, Functional Assay, Titration, Fluorescence, Clone Assay, Immunoprecipitation

other:

Article Title: ORY-1001 Delays Retinal Photoreceptor Degeneration in rd10 Mice by Inhibiting H3K4me2 Demethylation.
Article Snippet: The main antibodies used were anti-rhodopsin (catalog ab98887, Abcam, Cambridge, UK), anti-KDM1A (catalog 2184S, CST, USA), anti-H3K4me2 (catalog 9725S, CST, USA), anti-HDAC1 (catalog 34589S, CST, Danvers, MA, USA), anti-HDAC2 (catalog 57156S, CST, Danvers, MA, USA), anti-H3 (catalog 4499S, CST, Danvers, MA, USA), antiCoREST (catalog sc-376567, Santa Cruz, CA, USA), and anti-β-actin (catalog 66009-1-Ig, proteintech, Wuhan, China).

Article Title: MBD3 deficiency decommissions the NuRD complex and orchestrates the epigenetic regulation of gene expression to suppress neuroblastoma progression.
Article Snippet: 1 MBD3 deficiency decommissions the NuRD complex and orchestrates the epigenetic regulation of gene expression to suppress neuroblastoma progression Chen Chen, Bize Guo, Siyan Wu, Qinfang Zhu, Hui Shi, Jinhu Wang, Qiang Shu*, Ting Tao* Pediatric Cancer Research Center, National Clinical Research Center for Child Health, Children's Hospital Zhejiang University School of Medicine, Hangzhou, China (C.C., B.G., S.W., Q.Z., J.W., Q.S., T.T.); Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, China (H.S.); Department of Surgical Oncology, Children's Hospital Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China (J.W., T.T.); Zhejiang Key Laboratory of Neonatal Diseases, Hangzhou, China (J.W., Q.S., T.T.); Cancer Center, Zhejiang University, Hangzhou, China (J.W., T.T.). These authors contributed equally to this work.. *Corresponding Authors: Ting Tao, Pediatric Cancer Research Center, National Clinical Research Center for Child Health, Children's Hospital Zhejiang University School of Medicine, 3333 Binsheng Rd., Hangzhou 310052, China (taot@zju.edu.cn); Qiang Shu, Pediatric Cancer Research Center, National Clinical Research Center for Child Health, Children's Hospital Zhejiang University School of Medicine, 3333 Binsheng Rd., Hangzhou 310052, China (shuqiang@zju.edu.cn).

Article Title: ORY-1001 Delays Retinal Photoreceptor Degeneration in rd10 Mice by Inhibiting H3K4me2 Demethylation
Article Snippet: The main antibodies used were anti-rhodopsin (catalog ab98887, Abcam, Cambridge, UK), anti-KDM1A (catalog 2184S, CST, USA), anti-H3K4me2 (catalog 9725S, CST, USA), anti-HDAC1 (catalog 34589S, CST, Danvers, MA, USA), anti-HDAC2 (catalog 57156S, CST, Danvers, MA, USA), anti-H3 (catalog 4499S, CST, Danvers, MA, USA), anti-CoREST (catalog sc-376567, Santa Cruz, CA, USA), and anti- β -actin (catalog 66009-1-Ig, proteintech, Wuhan, China).

Article Title: HP1 enhances radio- and chemotherapy resistance in osteosarcoma via PARP1-dependent transcriptional repression and HR repair.
Article Snippet: Osteosarcoma is a primary malignant bone tumour with an unsatisfactory prognosis, and individualised and comprehensive treatment is needed.. HP1 (α/β/γ) is an important heterochromatin protein involved in transcriptional regulation and is also involved in the DNA damage response (DDR).. However, little is known about the participation of HP1 in DNA damage-induced transcriptional repression.

Article Title: Minute-scale coupling of chromatin marks and transcriptional bursts
Article Snippet: The primary antibodies used were anti-GAPDH (1:5000; Cell Signaling Technology, 5174, RRID:AB_10622025), anti-HDAC1 (1:1000; GeneTex, GTX100513, RRID:AB_1240929), anti-HDAC2 (1:1000; Cell Signaling Technology, 5113T, RRID:AB_10624871), and anti-HDAC3 (1:2000; GeneTex, GTX113303, RRID:AB_10721050).

Western Blot:

Article Title: ZNF787 recognizes the core sequence of MIR element to regulate gene expression
Article Snippet: Zinc finger (ZNF) proteins are widely studied as DNA-binding transcriptional regulators, yet many remain functionally uncharacterized—particularly in the context of repetitive genomic regions.. Here, we report that ZNF787 specifically binds a conserved 9-bp core motif within mammalian-wide interspersed repeats (MIRs) and interacts with the nucleosome remodeling and deacetylase (NuRD) complex.. Depletion of ZNF787 leads to de-repression of nearby genes accompanied by increased local H3K27ac levels.

Ubiquitin Proteomics:

Article Title: Proteasome activity maintains cell-type-specific gene expression.
Article Snippet: .. Antibodies used included anti-Rpn13 Pru (epitope against amino acids 100–200, ab157185, Abcam), anti-UCHL5 (ab133508, Abcam), anti-Rpn2 (ab2941, Abcam), anti-Rpn8 (ab140428, Abcam), anti-Rpt3 (ab140515, Abcam), anti-K48 ubiquitin (ab140601, Abcam), anti-CDK6 (Ab151247, Abcam), anti-SEC31A (ab86600, Abcam), anti-ubiquitin (3936, Cell Signaling Technology (CST)), anti-Rpn1 (25430, CST), anti-Rpn10 (epitope against N-terminal, 3336, CST), anti-Rpn10 (epitope against C-terminal, 12441, CST), anti-Rpn11 (4197, CST), anti-USP14 (11931, CST), anti-β-actin (4970, CST), anti-MGMT (2739, CST), anti-RAB25 (4314, CST), anti-p21 (2947, CST) anti-β5 (BML-PW8895-0100, ENZO), anti-E6AP (E8655, Sigma), anti-E6AP (ab290641, Abcam), antiOTUD5 (21002-1-AP-150UL, Proteintech), anti-OTUD5 (20087S, CST), anti-PALM3 (NBP2-81979, Novus biologicals LLC), antiSUSD2 (ab182147, Abcam), anti-UBR5 (65344S, CST), anti-HDAC2 (5113S, CST), and anti-S100A14 (10489-1-AP-150UL, Proteintech). .. HCT116 or HEK293T cells were obtained from American Tissue Culture Collection (ATCC) and authenticated by STR profiling prior to distribution, no additional authentication was performed.

Article Title: Proteasome activity maintains cell-type-specific gene expression
Article Snippet: .. Antibodies used included anti-Rpn13 Pru (epitope against amino acids 100–200, ab157185, Abcam), anti-UCHL5 (ab133508, Abcam), anti-Rpn2 (ab2941, Abcam), anti-Rpn8 (ab140428, Abcam), anti-Rpt3 (ab140515, Abcam), anti-K48 ubiquitin (ab140601, Abcam), anti-CDK6 ( Ab151247 , Abcam), anti-SEC31A (ab86600, Abcam), anti-ubiquitin (3936, Cell Signaling Technology (CST)), anti-Rpn1 (25430, CST), anti-Rpn10 (epitope against N-terminal, 3336, CST), anti-Rpn10 (epitope against C-terminal, 12441, CST), anti-Rpn11 (4197, CST), anti-USP14 (11931, CST), anti-β-actin (4970, CST), anti-MGMT (2739, CST), anti-RAB25 (4314, CST), anti-p21 (2947, CST) anti-β5 (BML-PW8895–0100, ENZO), anti-E6AP (E8655, Sigma), anti-E6AP (ab290641, Abcam), anti-OTUD5 (21002–1-AP-150UL, Proteintech), anti-OTUD5 (20087S, CST), anti-PALM3 (NBP2–81979, Novus biologicals LLC), anti-SUSD2 (ab182147, Abcam), anti-UBR5 (65344S, CST), anti-HDAC2 (5113S, CST), and anti-S100A14 (10489–1-AP-150UL, Protein-tech). .. Secondary antibodies used in this study included HRP-conjugated rabbit (A4914, Sigma or 31463, Thermo Fisher Scientific), mouse (A9917, Sigma) or Veriblot (ab131366, Abcam) antibodies.

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